Comparison of PCR-based high-resolution melting curve analysis and immunohistochemistry in detecting microsatellite instability in colorectal cancer: a retrospective consistency study.

Microsatellite instability (MS I) is an important biomarker for the prognosis and immunotherapy indications of colorectal cancer (CRC). Traditional detection methods such as immunohistochemistry (I HC) and polymerase chain reaction (PCR)-based capillary electrophoresis (PCR-CE) are widely used, but there are limitations such as strong subjectivity and time-consuming result judgment.

This study aims to evaluate the polymerase chain reaction-high-resolution melting curve (PCR-HRM) as a fast, high-throughput MS I detection method. Tumor samples of 251 CRC patients were analyzed. PCR-HRM and IHC methods are used to evaluate the status of MSI. If the results of PCR-HRM and IHC are inconsistent, the gold standard PCR-CE method is used for confirmation testing.

In this study cohort, 20.3% (51/251) of patients had MSI-H or mismatch repair defect (dMMR). PCR-HRM and IHC have excellent consistency, with an overall consistency rate of 99.20% and a Kappa coefficient of 0.971 (P